asc52telo (ATCC)
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asc52telo
Asc52telo, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/htert+asc52telo/pmc13278876-157-6-8?v=ATCC
Average 96 stars, based on 247 article reviews
Asc52telo, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/htert+asc52telo/pmc13278876-157-6-8?v=ATCC
Average 96 stars, based on 247 article reviews
asc52telo - by Bioz Stars,
2026-08
96/100 stars
Images
1) Product Images from "Live monitoring uncovers divergent epigenetic remodeling during osteogenic and adipogenic differentiation of mesenchymal stem cells"
Article Title: Live monitoring uncovers divergent epigenetic remodeling during osteogenic and adipogenic differentiation of mesenchymal stem cells
Journal: Frontiers in Cell and Developmental Biology
doi: 10.3389/fcell.2026.1823110
Figure Legend Snippet: The Impact of K9-MILo on the differentiation potential of ASC52telo cells. (a) Schematic representation of K9-MILo vector. (b) AlphaFold3-predicted structural model of K9-MILo. (c) RT-qPCR analysis of K9-MILo and endogenous MPP8 expression levels on day 0 of differentiation, normalized to RPLP0. Data are represented as Boxplot, n = 3, Mann–Whitney U test, * marks statistically significant differences (p < 0, 05) between ASC52telo versus the corresponding expression in ASC52telo-K9-MILo cells. (d) Differentiation of ASC52telo-K9-MILo cell line into osteocytes and adipocytes, right: Alizarin red staining of calcium deposits (red color); middle and left: Nile red staining of accumulated lipids (green fluorescence). Scale bar: 1,000 μm. (e) Quantification of differentiation efficiency. Top: Osteogenic differentiation was assessed by calculating the ratio of Alizarin Red S-positive pixels to total pixels in RGB images. Center and bottom: Adipogenic differentiation efficiency was evaluated by manual counting of cells containing lipid droplets within a 200 μm 2 area using ImageJ 1.54p (n = 4).
Techniques Used: Plasmid Preparation, Quantitative RT-PCR, Expressing, MANN-WHITNEY, Staining, Fluorescence
Figure Legend Snippet: Experimental and analytical pipeline for tracking heterochromatin dynamics during MSC differentiation. (a) Experimental workflow (left). A stable ASC52telo-MSC line constitutively expressing K9-MILo (generated via lentiviral transduction and bulk FACS sorting) is differentiated toward adipogenic or osteogenic lineages for up to 13 days. Every 48 h, live cells are imaged by widefield fluorescence microscopy (green channel). Automated XY positioning captures the same fields of view across all time points. Representative images show the nuclear distribution of H3K9me3 as a punctate heterochromatin pattern. (b) Analytical pipeline (right). Acquired nuclear images undergo segmentation. For each nucleus, 98 features are extracted (Haralick texture features, TAS, Zernike moments, chromatin distribution statistics). Feature vectors are averaged across 40 nuclei per condition per time point, then subjected to PCA. PC1 and PC2 are used to generate scatter plots revealing clustering of heterochromatin states.
Techniques Used: Expressing, Generated, Transduction, Fluorescence, Microscopy
Figure Legend Snippet: Evaluation of indomethacin impact on adipogenic differentiation of ASC52telo-K9-MILo. (a) Main signalling components in adipogenic differentiation, classical components of the differentiation cocktail are shown as dark green. (b) Adipogenic differentiation of ASC52telo, Nile red staining of accumulated lipids (green fluorescence) . (c) Principal component analysis of adipogenic differentiation in the presence and absence of indomethacin.
Techniques Used: Staining, Fluorescence